To view all 17 products belonging to this category, click on the link here.

Method Development Kits

Zorbax Products Have Unique Chemistry for Extended Column Lifetime at Different pH Ranges.

For convenience a number of Method Development Kits have been assembled to facilitate method development in different pH ranges.

Once the optimum Column type has been established then further refinements can be investigated with changes in Phase Selectivity e.g. C18 Vs C8 Vs Phenyl etc.

Fast (3.5µm) columns are also available to reduce run times without sacrificing Chromatographic Resolution.

Recent additions include Aqueous Method Development Kits for up to 100% Water Mobile Phases.

Further details of the different chemistries and their optimum pH Stability are detailed below.

For a FREE Method Development Poster or Application CD please contact agilent@carlstuart.com

Stable Bond

	Designed for low pH Sterically protected silanes Non-endcapped Highly temperature stable Short-chain phases very stable

ZORBAX SB-Aq

• Reproducible retention of polar analytes in aqueous mobile phases



• Excellent stability in low pH mobile phases



• Sterically protected silanes as per Stable Bond



• Complementary approach to ion-pair separations



• Usability with ion-pair reagents



• Rapid re-equilibration to initial aqueous conditions with gradients



• Alternative separation of biomolecules such as peptides, proteins and nucleotides

Exclipse XDB

Designed for mid-range pH Dimethylalkyl silanes Unique double-endcapping Uses silica sol - more stable at neutral and high pH

Bonus-RP

	Polar-linked alkyl phase - the ultimate in superb peak shape for basic compounds - over a wide pH range Long column life (pH 2-8) Unique selectivity Mild hydrophobic retention - Bonus-RP < C8 < C18 Patented bonding technology

– polar-linked alkyl phase for fast mass transfer giving good peak shape

– bulky side groups give low pH stability

– triple endcapped for mid-range pH stability and good peak shape

Zorbax Extend

• High life time pH 2 to 11.5



• Better peak shape and life time than polymer based columns



• Additional selectivity for basic compounds using high pH mobile phases



• Exhibits Less baseline noise with alkaline buffers and LC/MS

Zorbax Poroshell

A solid silica core with a superficially porous surface of smaller silica sol particles derivatized with bonded phase

• The Poroshell 300SB-C18 particle is a revolutionary particle that produces very fast, high resolution, RP-HPLC separation of proteins, DNA and other macromolecules. Many separations are fast, but Poroshell is both fast and exhibits high resolution.



• Poroshell particles can be derivatized with a variety of bonded phases. The first available product is Poroshell 300SB-C18.



• Poroshell works well for high resolution of macromolecules because of the rapid kinetics on and off of the thin porous shell bonded phase. Kinetics for macromolecules on completely porous particles, such as 3.5 micron particles, are slower due to the increased path the macromolecule must travel.



• Poroshell represents new technology and we are the only supplier of this unique product.