Partisil®

Through Whatman we offer a wide range of high performance liquid chromatography (HPLC) columns to meet your specific needs. In addition to the innovative Whatman Void Sealing Columns, we make available a selection of standard end fitting column configurations for your analytical and preparative needs. These are specifically designed for compatibility with all HPLC instrumentation.

The Whatman Partisil® is a high purity irregular silica gel, available in both 5 μm and 10 μm particle sizes, with a pore size of 80Å. The choice of column packing includes Silica, C-18 polymeric phases (ODS-3,ODS-2) and C-8. Also available are SAX (Strong Anion Exchanger), SCX (Strong Cation Exchanger), and PAC (Polar Amino Cyano). These columns provide reproducible results, column to column and lot to lot.

Due to the greater surface area of the irregular Partisil, the medium offers enhanced selectivity and loading capacity. Through uniform particle sizing, back pressure is minimized. Also, the neutral pH of Partisil provides for better peak symmetry without the need for mobile phase modifiers.

Partisil® Silica Media can be characterised as follows:

Adsorption (Normal Phase) Media
Ion Exchange Media
Reversed Phase Media
Adsorption (Normal Phase) Media

Partisil 5
Partisil 5 is a high efficiency stationary phase for adsorption chromatography that provides good selectivity and high loading capacity, for maximum resolution and fast analysis. Partisil 5 is available prepacked in Whatman Void Sealing (WVS) and Analytical and Rapid Analysis Column (RAC) configurations. Partisil 5 is the support on which the 5 μm bonded phases are based.

Partisil 10The stationary phase for routine separations, Partisil 10 is the support material for the 10 μm bonded media. This medium is used when higher flow rates are indicated and back pressure must be minimized. In addition to bulk media, it is available prepacked in most column configurations.

Ion Exchange Media

Partisil SAX (10 μm)
Partisil SAX (10 μm) is a strong anion exchanger based on quaternary ammonium groups (-NR3+). Supplied in the H2PO4 form in methanol, Partisil 10 SAX has been widely reported in literature and is best known for separation of nucleotides. Stable over pH range 1.5-7.5 when used in conjunction with a Solvecon mobile phase conditioning column, it obtains the highest anion exchange efficiencies and resolution.Partisil 10 SAX is applicable to separations of nucleic acids, organic acids, and inorganic anions. Please Contact Us for availability of specific combinations of columns.

Partisil SCX (5 μm and 10 μm)
Based on aromatic benzene sulfonic acid groups, Partisil SCX is supplied in the ammonium form (NH4+). Excellent for separation of nucleic acids, amino acids, polyamines, drugs, and other cationic species. Capable of being loaded with specific metallic cations for use in ligand exchange chromatography. Stable over pH range 1.5-7.0 when used in conjunction with a Solvecon mobile phase conditioning column. Exceptionally stable Si-O-Si-C bond, both thermally and chemically. Please Contact Us for availability of specific combinations of columns.

Reversed Phase Media

Partisil ODS (10 μm)
A C-18 phase with a 5%carbon load for both normal adsorption and reversed phase partitioning. Dual-mode operation for added selectivity with 50% residual silanols. Lightly loaded C-18 packing is particularly effective for compounds having greater water solubility when used in the reversed phase mode. Creates a moderately polar surface, different from that of pure silica, in normal phase mode. Please Contact Us for availability of specific columns.

Partisil ODS-2 (10 μm)
The high carbon load (16%) of this polymeric phase makes it the most nonpolar and, therefore, the most retentive of the reversed phases. An alternative to end-capped C-18 where different elution order is desirable for optimum separation. High sample load capacity and 10 μm particle size are very suitable for preparative work.

Partisil ODS-3 (5 μm and 10 μm)
A C-18 polymeric phase with a 10.5% carbon load. Medium of choice for improved speed, efficiency and resolution in applications requiring C-18 phases. End-capped for deactivation of silanols to minimize the need for ion suppression or ion pairing agents. Used in a wide range of applications with optimal selectivity, including pharmaceuticals, natural products, food, biological, and environmental pollutants.

Partisil C-8 (5 μm and 10 μm)
An end-capped C-8 monomeric phase with at least 8.5% carbon load. Provides high efficiency and rapid mass transfer while maintaining excellent peak shape and stability over a range of aqueous mobile phase compositions. Recommended for ion pair chromatography.

Partisil PAC (5 μm and 10 μm)
A polar amino cyano bonded phase, with secondary amine groups, for good thermal and chemical stability. Selectivity and rapid equilibrium allow a range of separation mechanisms to be used, including adsorption, reversed phase and weak anion exchange. Extremely fast equilibration across the entire range of solvents from heptane to water. The media of choice for carbohydrate separations.

Available Configurations

Standard Analytical : 4.6 mm ID x 25 cm long, standard analytical column for research, methods development and routine separations. After optimization, other sizes can be considered for greater speed or capacity. Allows direct scale-up or scale-down to other size columns. Supplied with Whatman compression screw end fittings
RAC II : 4.6 mm ID x 10 cm long. Second-generation Rapid Analysis Chromatography for faster analytical separations and reduced solvent consumption. Operates at low back pressure, even at high flow rates, prolonging column life. Connects easily to most LC instrumentation with convenient Whatman compression screw end fittings
Magnum 9 (50 cm) : 9.4 mm ID x 50 cm long. Semi preparative columns for microgram to gram quantities. Unique coned outlet allows high load capacity with minimal peak distortion. Durable construction ensures extended service. Magnum 9 columns are compatible with today's HPLC instruments, allowing you to use the same equipment for analytical and preparative work.
Magnum 9 (25 cm) : 9.4 mm ID x 25 cm long.
Magnum 20 (50 cm) :22 mm ID x 50 cm long. Preparative columns for multigram separations. Coned outlet allows high load capacity with minimal peak distortion. Durable construction ensures extended service. Magnum 20 columns provide sufficient yield and resolving power to accomplish difficult separations on a single pass, achieving high product purity
Magnum 20 (25 cm)
22 mm ID x 25 cm long